Efficient Protocol for In vitro Regeneration of Ocimum sanctum using Nodal Segments as Explants

Ocimum sanctum commonly called (holy basil) an herb containing medicinal, ornamental values, is often used in culinary applications. This research focuses on the improved and efficient protocol for direct regeneration acclimatisation of using nodal segments. Organogenesis multiplication from explants were observed to a maximum Murashige Skoog (MS) medium supplemented with 0.1 mg/L 6-Benzyl amino purine (BAP) 0.025 Indole-3-acetic acid (IAA). Furthermore, same was found effective induction roots, in-vitro grown plantlets. A series experiments conducted optimise rooted plantlets sanctum. For this study different types potting mix assorted ratios obtain best supporting media acclimatisation, A7 soil : farmyard manure (75:25) A1 (100%) sand hardening sanctum.